HPD antibody

Catalog No. ABIN2855744

The Rabbit Polyclonal anti-HPD antibody has been validated for WB. It is suitable to detect HPD in samples from Human. There is 1 publication available.

Quick Overview for HPD antibody (ABIN2855744)

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPD antibody is un-conjugated

Application: Western Blotting (WB)

Grade: KO Validated

Product Details anti-HPD Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to HPD (4-hydroxyphenylpyruvate dioxygenase)
HPD antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HPD. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HPD antibody (ABIN2855744)

Shan, Lu, Li, Sheng, Fan, Qi, Liu, Zhang: "4-hydroxyphenylpyruvate dioxygenase promotes lung cancer growth via pentose phosphate pathway (PPP) flux mediated by LKB1-AMPK/HDAC10/G6PD axis." in: Cell death & disease, Vol. 10, Issue 7, pp. 525, (2020) (PubMed).

Target Details for HPD

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Alternative Name: 4-hydroxyphenylpyruvate dioxygenase

Background: The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 45 kDa

Gene ID: 3242

UniProt: P32754